General Information

Sample Input

nCounter is compatible with most sample types, including even decades-old FFPE. It produces high quality data that would otherwise be difficult to produce with technologies that rely on high sample input. Consistent results can even be generated for longitudinal studies with a high degree of confidence on laboratory-grade samples requiring high-quality results.

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Required Input

Gene Expression, miRNA: 100ng purified total RNA or 300ng degraded

AZClinCore offers a variety of extraction services for most sample types, please inquire for details and pricing.

Expected Results

Gene Expression

Limit of Detection:
       •  0.5fM spike-in control (~1 copy per cell); 90% of the time
Fold-change Sensitivity:
       •  >1.5 fold (>5 copies per cell)
       •  >2 fold change (>1 copy per cell)

miRNA Assay

Limit of Detection:
       •  ≤ 0.5fM (~10 copies per cell)
Fold-change Sensitivity:
       • > 2 fold change

Details

Sample Preparation Details

Varies, please inquire.

Workflow

A variety of pre-designed panels are available for off-the-shelf use. Each cartridge can run up to 12 samples, and most panel kits are sold with chemistry for 12, 24, 48 or 96 samples (Custom Panels start at 48 samples). For a complete list of the number of samples and specific genes covered by each panel, please visit the Nanostring website. 

Custom CodeSets

For ultimate flexibility, you can work with NanoString directly (consultation is free of charge) to design a custom Gene Expression or Copy Number Variation code set tailored exactly to your research needs. This is especially useful for organisms that don't already have pre-built panels, hard-to-target sequences, and splice variation and fusion transcript detection. The custom CodeSet design process begins by selecting a list of targets and submitting them to NanoString. Once the design is approved by you, their Manufacturing Group will assemble your CodeSet, perform extensive quality control testing, and ship all components directly to the AZClinCore for processing. Custom CodeSet pricing is matrix based, with the price per data point declining with increasing numbers of genes and numbers of samples assayed.
 

Price

Academic (UA): $575.00 | Academic(non-UA): $690.00 | Industry: $862.50 | Unit: Cartridge

Note- Cost per Cartridge does not include QC, additional labor or NanoString Code Sets. Reagents must be purchased separately. Please inquire for more details.

Turnaround Time

Depends on assay type; please inquire.